Protein_Domain

Part:BBa_K112611:Design

Designed by: Sherine Cheung   Group: iGEM08_UC_Berkeley   (2008-10-22)


LamB pp> in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Construction of {LamB>} basic part K112611 

        
PCR cb10009/sc014 on MG1655                   (107 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

References